Fig. 5
From: Efficient prioritization of CRISPR screen hits by accounting for targeting efficiency of guide RNA
sgRNA activity-corrected viability metric v more efficiently identifies essential genes. A Druggable gene library CRISPR-Cas9 screening workflow. B The moving median of all sgRNAs according to X analyzed by FC or v. C The receiver operating characteristic (ROC) curve of three analytic methods. The true positive rate is decided by essential genes and plotted against the false positive rate, decided by non-essential genes (left). The area under the curve analysis for essential (solid line) and non-essential (dotted line) genes (right). D The receiver operating characteristic (ROC) curve of CRISPR screening results of Brunello library using three analytic methods. E Calculated ROC-AUC values from D. F Recall percentage at 20% false discovery rate (FDR) calculated by three methods. G False discovery rate (FDR) recall at 95%, 97.5%, and 98% calculated by three methods