Fig. 7
From: Hypoxia induces alterations in tRNA modifications involved in translational control
Functional analysis of total tRNA from NC and hypoxia groups’ testis in GC-2spd cells. A Schematic diagram of testis total tRNA-enriched fragment transfection into GC-2spd cells. B Nascent protein synthesis of GC-2spd cells transfected with total tRNA-enriched fragments from NC and Hypo group mouse testis. C Growth analysis of GC-2spd cells through counting cell number at 48 h after transfection and CCK8 at pointed time after transfection (n = 3~7, Additional file 13). D Expression levels of individual tRNAAla and tsRNAAla in GC-2spd cells transfected with total tRNA-enriched fragments from NC and Hypo group mouse testis after 48 h. E Western blot analysis of eIF4A and eIF4E expression in GC-2spd cells at 48 h transfected with total tRNA between NC and Hypo groups from mouse testis. **p < 0.01, ***p < 0.001, ****p < 0.0001; NB, northern blot. All results are shown as mean ± SEM. Statistical analysis was conducted using one-way ANOVA with uncorrected Fisher’s LSD