Fig. 2.

Analysis of the folding and dimerization of drNurA. A The overview of the cryo-EM analysis of drNurA-HerA. Upper left, an example of a typical micrograph showing particle distribution of the drHerA-NurA complex. Lower left, a selection of 2D class averages representing different particle views. Middle, the side view of the model of the drNurA-HerA complex fitted into the cryo-EM map. Right, the top view of the model of the drNurA-HerA complex fitted into the cryo-EM map. B Gel filtration analysis of the dimerization of drNurA. Wild-type drNurA and drNurA with β* (the N-terminal seven residues) deleted mutant (drNurAdN7) were analyzed with Superdex S200 HR 10/300 column. Peaks of each injection were reconstructed using GraphPad Prism software. Fractions eluted were resolved by 12% SDS-PAGE. C Left, the topology diagram of bacterial NurA. The catalytic sites are shown as green dots. The potential drNurA-HerA interaction motifs are numbered and colored orange. The inserted motif (a β-turn) in bacterial NurA is colored red. Right, the bottom view of drNurA dimer. The catalytic sites are labeled as sticks and colored green. The dimerization area on drNurA is squared and the zoom-in view from another orientation is shown by the side. D Left, the topology diagram of archaeal NurA. The inserted motifs are shown as grey squares. Right, the bottom view of the ssoNurA (PDB ID: 2YGK) dimer