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Table 2 ATPase analysis of drNurA-HerA and mutant

From: Structural and DNA end resection study of the bacterial NurA-HerA complex

HerAb

NurAb

ATPase mol/protein/min

(no DNA)

ATPase mol/protein/min

(ss DNA)

ATPase mol/protein/min

(ds DNA)

wta

-

<<0.01

<<0.01

<<0.01

-

wta

<<0.01

<<0.01

<<0.01

wta

wta

0.08±0.03

0.52±0.09

0.45±0.07

wt

wt

0.42±0.23

28.63±1.78

4.18±0.36

wt

-

<<0.01

1.69±0.09

0.09±0.04

-

wt

<<0.01

<<0.01

<<0.01

HerAm

wt

<<0.01

6.01±1.54

0.38±0.09

487/8A

wt

<<0.01

<<0.01

<<0.01

495A

wt

0.46±0.15

6.53±1.11

0.72±0.14

552A

wt

0.39±0.21

9.68±1.81

0.86±0.19

96A

wt

0.45±0.17

27.93±1.99

4.01±0.46

wt

53A

0.43±0.09

24.23±2.38

3.04±0.45

wt

201A

0.46±0.13

21.11±1.98

3.81±0.39

wt

309A

0.34±0.12

28.31±2.71

2.98±0.51

wt

dPin

0.27±0.02

15.85±2.04

0.10±0.04

wt

8A

0.36±0.16

26.64±1.76

0.28±0.08

wt

13A

0.39±0.11

27.13±1.01

3.94±0.19

  1. aindicate Mg2+ was used instead of Mn2+ in this assay
  2. b indicate the addition of wild type or mutated proteins
  3. The measurements were repeated more than six times