Fig. 5

SRSF1 deficiency impairs chromosome synapsis and the formation of COs at meiotic prophase I. a Co-immunostaining of MLH1 and SYCP3 in newborn Ctrl and cKO oocytes. DNA was stained with DAPI. Scale bar, 5 μm. b The number of COs, marked by MLH1 foci (red), was significantly reduced in cKO diplotene-like oocytes compared with Ctrl oocytes. Twenty-five Ctrl oocytes and thirty-three cKO oocytes were obtained from 3 animals. Significance was determined by unpaired Student’s t test; ****P < 0.0001. The error bar represents the mean ± SEM. c Localization of SYCP1, SYCP3, and CREST in newborn Ctrl and cKO oocytes. DNA was stained with DAPI. Scale bar, 5 μm. Diagrams illustrate aberrant synapsis in Ctrl (d) and cKO (e) oocytes