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Fig. 6 | BMC Biology

Fig. 6

From: SRSF1 regulates primordial follicle formation and number determination during meiotic prophase I

Fig. 6

SRSF1 is necessary for the resection of DSBs. a Co-immunostaining of γH2AX and SYCP3 in 18.5 dpc Ctrl and cKO ovaries. DNA was stained with DAPI. Scale bar of the top panel, 50 μm. Scale bar of the rest panel, 10 μm. b Percentage of double-positive cells is shown as the mean ± SEM in 18.5 dpc ovaries. Ctrl, n = 6; cKO, n = 5. c Co-immunostaining of RPA1 and SYCP3 in newborn Ctrl and cKO oocytes. DNA was stained with DAPI. Scale bar, 5 μm. d In cKO diplotene-like oocytes, the number of RPA1 foci (red) was maintained at a relatively high level. Twenty-five Ctrl oocytes and thirty-three cKO oocytes were obtained from 3 animals. e Localization of DMC1, γH2AX, and SYCP3 in 18.5 dpc Ctrl and cKO oocytes. DNA was stained with DAPI. Scale bar, 10 μm. f In cKO pachytene oocytes, the number of DMC1 foci (white) was maintained at a relatively high level. Sixty Ctrl oocytes and fifty-six cKO oocytes were obtained from 4 animals. Significance was determined by unpaired Student’s t test; detailed P value P ≥ 0.05, *P < 0.05, ***P < 0.001, ****P < 0.0001. The error bar represents the mean ± SEM

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