Fig. 1
From: Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling
Characteristic differences between pre-diapause and diapause embryos. A Morphological examination of the blastoderm at different states. B, C Amount of extracted RNA (B) and DNA (C) from each embryo. D Total cell number counted by flow cytometry. E The diameter of blastoderms. F Immunostaining of blastoderm sections with an antibody against the mitosis marker phospho-Histone H3 (H3S10P). Samples were counterstained with DAPI to visualize DNA (blue). Scale bar, 100 μm. G, Box plots show the abundance of representative metabolites at F0 or S8d blastoderms. Solid lines in the boxes indicate the median abundance, and the + symbols indicate the mean abundance. The VIP value obtained by OPLS-DA and the p value obtained by Student’s t test are provided. See also Additional file 1: Fig. S1C