Fig. 2
From: Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-κB-IRF1 signaling
Overview of transcriptomic changes during the onset and maintenance of diapause. A Schematic diagram depicting the RNA-Seq workflow time course. Blastoderms isolated from fresh oviposited eggs and low temperature-stored eggs represent pre-diapause and diapause embryos, respectively. Embryos in the diapause state are indicated as S1d, S2d, S4d, S8d and S12d, corresponding to 1, 2, 4, 8 and 12 days of storage, respectively. B PCA displays overall gene expression profiles across samples in (A). C Number of DEGs identified between blastoderms isolated from fresh oviposited eggs and low temperature-stored eggs. DEGs were screened under the threshold of an FDR < 0.01 and |log2fold change| >1. The red and blue lines show the numbers of upregulated and downregulated genes, respectively. The dashed line shows the number of DEGs normalized by the number of interval days. D, E GO enrichment (D) and KEGG pathway (E) analysis of DEGs. F Number of annotated genes that undergo negative or positive regulation of cell proliferation. G A summary of DEGs annotated for negative or positive regulation of cell proliferation