Fig. 5

DGD1-carrying CML235 growth curves and Dgd1 protein production. A The cells were cultured in 200 µL of minimal medium (YNB, 10 mM AIB, 10 mM proline, 1% raffinose as carbon source, and 2% galactose as inducer) at 25 °C. On the right, a plot of the maximum specific growth rates (µmax) is shown. The error bars are standard deviations based on three biological replicas. Statistical differences were obtained through an ANOVA analysis. The raw data, Gompertz model fitting, and statistical analyses are given in Additional file 11. B Protein production profile at different time points after overnight cultures were transferred to the inducer medium. The V5-labelled Dgd1p and lacZ proteins were detected by horseradish peroxidase (HRP)-conjugated anti-V5 primary antibody. The loading protein control Tdh3p was detected by Anti-Tdh3p primary antibody coupled with an HRP-conjugated anti-mouse secondary antibody. Protein production is equal to the Dgd1p/LacZ peak band area divided by the Tdh3p peak band area. The Western blot raw data, the experimental molecular weight of the Dgd1p produced by the expression of the S. uvarum and S. kudriavzevii DGD1 alleles, and the molecular weight calibration are given in Additional file 11