Fig. 9

DGD1 is present in other Saccharomycotina yeasts. A DGD1 phylogenetic tree, inferred by using the maximum likelihood method conducted in MEGA 11, based on the LG model. The tree with the highest log likelihood (− 11,476.87) is shown. A discrete Gamma distribution was used to model evolutionary rate differences among sites (+ G, parameter = 0.5623). The tree is drawn to scale, with branch lengths measured in the number of amino acid substitutions per site. Numbers on the nodes correspond to bootstrap values based on 500 replicates. Circles of different colors at the right of the yeast names indicate the phylogenetic clades they belong to according to previous yeast phylogenetic analyses and taxonomic proposals [4, 37]. B Growth phenotype of Zygosaccharomyces and Torulaspora isolates in the presence of AIB as the sole nitrogen source