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Fig. 1 | BMC Biology

Fig. 1

From: Synchronization of inspiratory burst onset along the ventral respiratory column in the neonate mouse is mediated by electrotonic coupling

Fig. 1

Features of the preparation. A.i The average of network activity during individual inspiratory burst reveals synchronized activity along the VRC; salience of Ca2+ signals are enhanced by subtracting each image from the image acquire 1.7 s earlier. Intersecting dashed red lines indicate the origin used to align datasets, defined as the caudal pole of the VIIn (dashed white arc) and the minimum of the ventral surface concavity caudal to VIIn. ROIs overlapping with preBötC (blue), PiCo (red), and pFRG/RTN (green) are superimposed A.ii Traces associated ROIs in preBötC (blue traces), in PiCo (red traces), and pFRG/RTN (green traces) reveals stereotypy of motor output along the VRC. B ROI centers of mass from one germline-GCaMP6F dataset (orange points) and an inducible-Cre dbx1-GCaMP6F exposed to tamoxifen at E9.5 to selectively express GCaMP6F in preBötC neurons (black points) are overlayed on collated data from 180 s (N = 11, 1285 ROIs) and 600 s (N = 11, 1534 ROIs) high-speed (100 Hz) optical recordings, shown in gray. C Merged maximum intensity images obtained from a 450 μm slice sectioned from the preparation shown in A, processed for phox2b (green), SST (red), and ChAT (blue). After rescaling, immunoprocessed and fresh tissue were aligned using optical signals and vasculature (video in Additional file 1). D ROIs superimposed on images from immunoprocessed tissue reveal preBötC ROIs are in a region with sparse phox2b and ChAT expression but high SST expression (D.i). By contrast, PiCo ROIs are located in a region with few phox2b+ ChAT+ or SST+ neurons (D.ii). ROIs in pFRG/RTN overlapped with phox2b+ and ChAT+ networks but overlap with SST+ neurons was low (D.iii)

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