Skip to main content
Fig. 1 | BMC Biology

Fig. 1

From: Cytological, transcriptome and miRNome temporal landscapes decode enhancement of rice grain size

Fig. 1

Variation in seed morphology and seed development amongst SN and LGR. A Mature seeds of SN and LGR arranged width-wise (top panel) and length wise (right panel, scale bar = 2 cm) and an equal number of mature dry husked seeds of SN and LGR, (scale bar = 1 cm) arranged to resemble a rice seed, show LGR seeds are bigger in size and cover more area. Red box represents middle seed portion used for SEM analysis. Graphs representing B length (mm) and C width (mm) of a mature seed of SN and LGR (n = 100). D Average 1000-seed weight (grams) of mature dried seeds (n = 3) (See replicate data in Additional file 2: Table S18). Graphs showing E cell number, F cell length (μm), and G cell area (μm2) from middle portion of SN and LGR husks (n = 6*3). Asterisks are as determined by Student’s t tests, one-sided (*, **, *** = p value ≤ 0.05, ≤ 0.01, ≤ 0.001, respectively). H Grain filling rate of SN and LGR. Bars represent total weight of 15 seeds from each stage (n = 3) (See replicate data in Additional file 2: Table S18). Dotted lines indicate per cent increase/decrease in seed weight in one stage with respect to previous stage. The triangles and squares represent SN and LGR, respectively. Error bars represent ± SD in all the graphs. I Endosperm sections of SN (left) and LGR (right) at selected DAP, representing S2, S3, and S4 stages of seed development stained with toluidine blue-O. i, v, ix, xiii, xvii and iii, vii, xi, xv, xix show central endosperm of SN and LGR, respectively, while ii, vi, x, xiv, xviii and iv, viii, xii, xvi, xx show peripheral endosperms of SN and LGR, respectively. i-iv, v-viii, ix-xii, xiii-xvi and xvii-xx represent 4, 5, 7, 9, and 11 DAP (as labelled). Red and blue triangles indicate nuclei and cell wall, respectively. Scale bar = 50 µm

Back to article page