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Fig. 4 | BMC Biology

Fig. 4

From: Cytological, transcriptome and miRNome temporal landscapes decode enhancement of rice grain size

Fig. 4

Carbohydrate metabolism in seed stages (S1–S5) and flag leaf (Leaf) of SN and LGR. A, B Heat map showing expression levels (log2FPKM) of genes related to carbohydrate biosynthesis (SY, two clusters) and degradation (DG, four clusters), respectively. C KI/I2 staining of the endosperm sections of SN and LGR. The representative DAP from three stages of seed development have been mentioned on the left side and corresponding stages have been mentioned below the DAP. Left and right panels show sections of SN and LGR seeds, respectively (as indicated on the top). Scale bar = 50 µm. D Starch estimation in different seed developmental stages of SN & LGR (n = 2). The five stages of seed development are represented on the X-axis. The Y-axis shows the amount of starch in grams per 100 g of seed. Blue line represents SN while red line represents LGR. Standard error bars have been shown (See replicate data in Additional file 2: Table S18). E Three sucrose synthase genes showed high expression (log2FPKM ≥ 5) in seed tissues of SN (upper panel) and LGR (lower panel). LOC_Os06g09450 and LOC_Os07g42490 showed peak expression in LGR S3, S4 and SN S2, S3, respectively, as marked with arrows

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