Fig. 7

In vitro and in vivo study of the mechanism of P. distasonis repairing intestinal barrier. A Relative mRNA expression of AhR and its target gene, CYP1A1 in colon tissue of rats (n = 8 per group). Each dot shows the value from each independent replicate. B Representative Western blot and densitometric analyses of AhR protein in colon tissue of rats (n = 6 per group). Each dot shows the value from each independent replicate. C The level of IL‑22 in the plasma was measured by ELISA (n = 8). Each dot shows the value from each independent replicate. D Relative mRNA expression level of Claudin-1, Occludin, and ZO-1 proteins in Caco-2 cells. Each dot shows the value from each independent replicate; E Representative Western blot and densitometric analyses of Claudin-1, Occludin, and ZO-1 protein in Caco-2 cells. Each dot shows the value from each independent replicate. F Representative pictures of immunofluorescence staining of MUC2 protein in Caco-2 cells (Green is FITC immunofluorescence staining of MUC2, and blue is DAPI staining of nucleus). Data are expressed as mean ± SEM. Differences were assessed by the Mann–Whitney U test. Significance was established at adjusted P < 0.05 with a false discovery rate (FDR) of 0.05