Fig. 5

Genomic collinearity, expression and binding activities of self-incompatibility genes across the two species. a Genomic collinearity of three self-incompatibility genes. b Expressions of three self-incompatibility genes in flowers (significance was tested by paired t test *P-value < 0.05). c The differences in the regulatory regions between the two species. d Transient expression assay of luminescence intensity show the different transcription activity of S-RNase in F. tataricum and F. esculentum. The color bar on the right represents the intensity of fluorescence. Representative images of N. benthamiana leaves 72 h after infiltration were shown