Fig. 2
From: Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication
Knockdown of foi leads to zinc deficiency in the fat body, and dietary zinc intervention modified a spectrum of phenotypes associated with foi knockdown. A Alkaline phosphatase (ALP) activity was significantly decreased in the fat body of Cg-Gal4 > foi RNAi third instar larvae and slightly increased in Cg-Gal4 > foi OE larvae. n = 40 fat bodies per group. Quantification from three independent experiments. All values are presented as mean ± SEM and included in Additional file 2. B Q-RT-PCR analysis of MtnB transcript levels showed that the expression of MtnB in the fat body was down-regulated in Cg-Gal4 > foi RNAi and upregulated in Cg-Gal4 > foi OE third instar larvae. RNA was made from the third instar larvae’s fat body. rp49 was used as control. n = 40 fat bodies per group. Quantification from three independent experiments. All values are presented as mean ± SEM and included in Additional file 2. C The eclosion rate of Cg-Gal4 > foi RNAi larvae could be rescued by dietary zinc supplementation. Cg-Gal4 was crossed to wild-type, foi RNAi or foi OE flies on juice-agar plates. Newly hatched progeny were transferred to normal food (NF) or food supplemented with zinc (2 mM ZnCl2) or zinc chelator (25 μM N, N, N’, N’-tetrakis (2-pyridylmethyl) ethylenediamine [TPEN]). Percentages of flies that eclosed to adults were counted. n = 50–70 larvae per vial, n = 6 vials per experimental group. D The reduced fat body size of Cg-Gal4 > foi RNAi larvae could be rescued by dietary zinc supplementation (2 mM ZnCl2) but exacerbated by dietary zinc depletion (25 μM TPEN) in food. n = 6 replicates per group. E The reduced fat body cell size of Cg-Gal4 > foi RNAi larvae could be rescued by dietary zinc supplementation (2 mM ZnCl2) and exacerbated by dietary zinc depletion (25 μM TPEN) in food. n = 6 replicates per group. Scale bar, 100 μm. F Quantitative measurement of the fat body cell sizes in (E). Genotypes used in (A-F) were Cg-Gal4 > w1118 (control), Cg-Gal4 > foi RNAi or Cg-Gal4 > foi OE. n = 6 replicates per group in (C-F). Results are presented as mean ± SEM of the biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001; two-tailed Student’s t-test. OE, overexpression