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Fig. 4 | BMC Biology

Fig. 4

From: Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication

Fig. 4

The developmental defect of foi RNAi is related to DNA endoreplication. A Autophagy inhibitor chloroquine (CQ) did not affect the eclosion defect of Cg-Gal4 > foi RNAi larvae. n = 50–70 larvae per vial, n = 6 vials per experimental group. B The fat body developmental defect of Cg-Gal4 > foi RNAi larvae was not rescued by CQ. Green line marks present the fat body tissues. n = 6 replicates per group. Genotypes used in (A-B) were Cg-Gal4 > w1118 (control), Cg-Gal4 > foi RNAi. C The eclosion defect of Cg-Gal4 > foi RNAi larvae was not affected by the apoptosis inhibitor p35. n = 50–70 larvae per vial, n = 6 vials per experimental group. D The fat body developmental defect of Cg-Gal4 > foi RNAi larvae showed no significant change in the presence of the apoptosis inhibitor p35. The green line marks present the fat body tissues. n = 6 replicates per group. Genotypes used in (C-D) were Cg-Gal4 > w1118 (control), Cg-Gal4 > p35, Cg-Gal4 > foi RNAi, Cg-Gal4 > p35; foi RNAi. E Nuclei double stained with 4’,6-diamidino-2-phenylindole (DAPI, blue) and 5-Ethynyl-2'-deoxyuridine (EdU, green) showed that the nuclei of Cg-Gal4 > foi RNAi display abnormal shape, smaller size and weaker fluorescence signals compared with control nuclei (Cg-Gal4 > w1118). n = 6 replicates per group. Scale bar, 50 μm. F The replication signals of EdU-positive cells in control, foi RNAi, or foi OE larvae fat body tissues were determined for the experiment in (E). n = 6 replicates per group. (control, n = 88; Cg-Gal4 > foi RNAi, n = 97; Cg-Gal4 > foi OE, n = 128 nuclei). Genotypes used in (E–F) were Cg-Gal4 > w1118 (control), Cg-Gal4 > foi RNAi, Cg-Gal4 > foi OE. Data are represented as mean ± SEM of the biological replicates. ***p < 0.001; two-tailed Student’s t-test. OE, overexpression

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