Fig. 5
From: Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication
The growth arrest, fat body developmental defects and blocked endoreplication in foi RNAi were restored by Myc overexpression. A Western blot analysis shows that Myc synthesized in the fat body decreased when foi was knocked down. Gapdh was used as the loading control. n = 40 fat bodies per group. B Quantitative measurement of (A). Quantification from three independent experiments. Genotypes used in (A-B) were Cg-Gal4 > w1118 (control), Cg-Gal4 > foi RNAi, Cg-Gal4 > foi OE. All values are presented as mean ± SEM and included in Additional file 2. C The eclosion defect of Cg-Gal4 > foi RNAi was almost fully rescued by Myc OE. n = 50–70 larvae per vial, n = 6 vials per experimental group. D The smaller fat body size and cell size of Cg-Gal4 > foi RNAi were significantly rescued by Myc OE. n = 6 replicates per group. Scale bar, 100 μm. E Quantitative measurement of the fat body cell sizes in (D). n = 6 replicates per group. F The endoreplication defects observed in Cg-Gal4 > foi RNAi fat body nuclei were significantly rescued by Myc OE. n = 6 replicates per group. Scale bar, 100 μm. G Quantitative measurement of the nuclei size in (F). (control, n = 64; Cg-Gal4 > foi RNAi, n = 125; Cg-Gal4 > Myc OE; foi RNAi, n = 73; Cg-Gal4 > Myc OE, n = 86 nuclei). H Quantitative measurement of the replication signals in (F). (control, n = 42; Cg-Gal4 > foi RNAi, n = 91; Cg-Gal4 > Myc OE; foi RNAi, n = 82; Cg-Gal4 > Myc OE, n = 78 nuclei). Genotypes used in (C-H) were Cg-Gal4 > w1118 (control), Cg-Gal4 > foi RNAi, Cg-Gal4 > Myc OE; foi RNAi, Cg-Gal4 > Myc OE. Data are represented as mean ± SEM of the biological replicates. *p < 0.05, ***p < 0.001; two-tailed Student’s t-test. OE, overexpression