Fig. 6
From: Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication
The decreased expression of Myc in foi RNAi is mediated by the JNK signaling pathway. A Western blot analysis shows that expression of phosphorylated JNK (pJNK) was activated in foi RNAi larvae in comparison to that of control. n = 40 fat bodies per group. B Quantitative measurement of (A). Quantification from three independent experiments. All values are presented as mean ± SEM and included in Additional file 2. C The increased expression of pJNK in Cg-Gal4 > foi RNAi larvae fat body was partially suppressed by dietary zinc supplementation. n = 40 fat bodies per group. D Quantitative measurement of (C). Quantification from three independent experiments. Genotypes used in (A-D) were Cg-Gal4 > w1118 (control), Cg-Gal4 > foi RNAi, Cg-Gal4 > foi OE. All values are presented as mean ± SEM and included in Additional file 2. E The decreased expression of Myc in foi RNAi larvae fat body could be significantly restored by JNK signaling inhibition, which is achieved by expression of a dominant negative form of the basket (bskDN). n = 40 fat bodies per group. Genotypes were Cg-Gal4 > w1118 (control), Cg-Gal4 > foi RNAi, Cg-Gal4 > bskDN; foi RNAi, Cg-Gal4 > bskDN. F Dietary zinc supplementation significantly increased Myc expression in the fat body of Cg-Gal4 > foi RNAi larvae. n = 40 fat bodies per group. Genotypes were Cg-Gal4 > w1118 (control), Cg-Gal4 > foi RNAi. Data are represented as mean ± SEM of the biological replicates. *p < 0.05, **p < 0.01; two-tailed Student’s t-test. OE, overexpression