Fig. 7
From: Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication
The growth arrest, fat body developmental defects and blocked endoreplication in foi RNAi could be rescued by JNK signaling inhibition. A The eclosion defect of Cg-Gal4 > foi RNAi was almost fully rescued by bskDN. n = 50–70 larvae per vial, n = 6 vials per experimental group. B The smaller fat body size and cell size of Cg-Gal4 > foi RNAi were rescued very well by bskDN. n = 6 replicates per group. Scale bar, 100 μm. C Quantitative measurement of the fat body cell sizes in (B). D The endoreplication defects observed in Cg-Gal4 > foi RNAi fat body nuclei were significantly rescued by bskDN. n = 6 replicates per group. Scale bar, 100 μm. E Quantitative measurement of the nuclei size in (D). (control, n = 141; Cg-Gal4 > foi RNAi, n = 108; Cg-Gal4 > bskDN; foi RNAi, n = 51; Cg-Gal4 > bskDN, n = 100 nuclei). F Quantitative measurement of the replication signals in (D). (control, n = 100; Cg-Gal4 > foi RNAi, n = 106; Cg-Gal4 > bskDN; foi RNAi, n = 96; Cg-Gal4 > bskDN, n = 80 nuclei). Genotypes used in (A-F) were Cg-Gal4 > w1118 (control), Cg-Gal4 > foi RNAi, Cg-Gal4 > bskDN; foi RNAi, Cg-Gal4 > bskDN. Data are represented as mean ± SEM of the biological replicates. ***p < 0.001; two-tailed Student’s t-test