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Fig. 8 | BMC Biology

Fig. 8

From: Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication

Fig. 8

Knockdown of foi in the fat body leads to ROS accumulation which could be rescued by dietary zinc supplementation. A 20, 70-dichlorodihydro fluorescein diacetate (DCFH-DA) staining showed that foi RNAi in the fat body leads to ROS accumulation (green signals). Zinc supplemented in the food could rescue the ROS accumulation. n = 10 fat bodies per group. Scale bar, 100 μm. B Quantitative measurement of (A). n = 5 replicates per group. All values are presented as mean ± SEM and included in Additional file 2. C Dihydroethidium (DHE) method indicated that abundant ROS was generated in the fat body upon foi RNAi and decreased when zinc supplemented in the food. n = 10 fat bodies per group. Scale bar, 100 μm. D Quantitative measurement of (C). n = 5 replicates per group. All values are presented as mean ± SEM and included in Additional file 2. Genotypes used in (A-D) were Cg-Gal4 > w1118 (control), Cg-Gal4 > foi RNAi. Data are represented as mean ± SEM of the biological replicates. *p < 0.05; ***p < 0.001; two-tailed Student’s t-test

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