Fig. 3
From: Regulation of Miwi-mediated mRNA stabilization by Ck137956/Tssa is essential for male fertility
Gene expression analysis of Ck137956 knockout pachytene spermatocytes and round spermatids. A Number of differentially expressed genes in Ck137956−/− pachytene spermatocytes and round spermatids. PAC, pachytene spermatocytes; RS, round spermatids. B Venn diagram showing overlap between down-regulated genes in Ck137956−/− pachytene spermatocytes and those in Ck137956−/− round spermatids. C Heatmap of genes with differential expression (Log2FoldChange > 2 and adjusted P < 0.01) between Ck137956+/- and Ck137956−/− mice in pachytene spermatocytes or round spermatids. D Heatmap of gene expression during spermatogenesis for genes down-regulated in Ck137956−/− RS. 2C: spermatogonia and somatic cells; LZ: leptotene/zygotene spermatocytes; PAC, pachytene spermatocytes; RS, round spermatids. E–F Enriched cellular component terms (E) and biological process terms (F) in the down-regulated genes in Ck137956−/− round spermatids. G Western blot analysis of Ck137956 expression in cytoplasm, nuclei, and chromatin. Gapdh, Hdac1 and histone H4 served as cytoplasm, nuclei and chromatin controls, respectively. H-I qRT-PCR analysis of the levels of mRNAs (H) and pre-mRNAs (I) of spermatogenesis-related genes in day-24 Ck137956+/- and Ck137956−/− testes. n = 3. Data were presented as the mean ± SEM. NS, not significant, **p < 0.01, ***p < 0.001. J Scatterplot of Log2FoldChange between transcriptome and proteome after Ck137956 deletion in round spermatids