Fig. 1

Downregulating gene expression using NgAgo/gDNA. A Survival rate of embryos after injection of gDNA and NgAgo mRNA. Experiments were performed in triplicate and repeated three times. The bars display mean ± se. Student’s t test was performed and n.s. indicates the difference is not statistically significant compared to control. n = 3 batches of injected embryos. B Specific alteration of morphology of zebrafish embryos after coinjection of NgAgo mRNA and fn1a-gDNA, observed at 22 hpf. The phenotype could be rescued by overexpressing fn1a mRNA. C Comparison of phonotypes generated by morpholino oligonucleotides and NgAgo/gDNA. (a) Representative NgAgo/pax6a-gDNA phenotype and (b) representative morphant phenotype of pax6a at 48 hpf compared to that of controls. D Phenotype of NgAgo-gDNA knockdown of dhx34 in zebrafish at 24 hpf. (a) Wild-type phenotype. (b) Phenotype after NgAgo treatment. (a' and b’) are the enlarged images of the trunk in Panels a and b, and the asterisks (*) indicate the somites. E, F Phenotype of NgAgo-gDNA knockdown of appa (E) and slc2a2 (F) in zebrafish at 36 hpf. The arrow indicates the malformed brain. G Statistical analysis of the knockdown efficiency of different targeted strands. n = 4