Fig. 2

Tuning NgAgo/gDNA by target position and GC content of gDNAs to effectively knock down target genes. A Schematic diagram of gDNA design on kat2a and slc2a2 in zebrafish. B Efficiency of downregulation of gDNAs at the 5’, middle and 3’ part of kat2a or slc2a2 pre-mRNA and mRNA, measured by qRT‒PCR at 24 hpf. qRT-PCR experiments were performed biologically three times. The bars display mean ± se. Student’s t test was performed and the ** indicated the difference compared to control is statistically significant (p < 0.01). n = 3. C, D The effect of the GC ratio of gDNAs targeting neighboring loci in kat2a and slc2a2. qRT-PCR experiments were performed biologically four times. The bars display mean ± se, and Student’s t test was performed and the different letters (a-d) indicate statistically significant differences (P < 0.05). n = 4