Fig. 4
From: Efficient manipulation of gene expression using Natronobacterium gregoryi Argonaute in zebrafish
NgAgo targets genomic DNA in the nucleus. A Localization of NLS-NgAgo-EGFP and NES-NgAgo-EGFP in HEK293T cells. Scale bar = 10 μm. B Knockdown efficiency of NLS-NgAgo and NES-NgAgo on mc4r, kat2a, and slc2a2. Experiments were performed biologically four times. The bar displays mean ± se, and Student’s t test was used and the ** indicated the difference compared to control is statistically significant (p < 0.01). n = 4. C, D Upregulation of kat2a expression using NLS-NgAgo-VP64 guided by gDNAs. n = 3. E, F Upregulation of appa expression using NLS-NgAgo-VP64 guided by gDNAs. n = 4. The qRT‒PCR experiments were performed biologically three times. The bars display mean ± se, and Student’s t test was used and the ** and * indicated the difference compared to control is statistically significant (p < 0.01 and p < 0.05, respectively)