Fig. 3

Immunodetection of Aristaless1 in wild-type and -Al1 CRISPR Heliconius cydno embryos. A Immunodetection of Al1 in wild-type embryos. White boxes highlight the mandibula (B), thoracic legs (C), and abdominal legs (D) zones shown at a higher magnification in the next panels. E–F Immunodetection of Al1 in injected -Al1 CRISPR embryos. G Higher magnification of the abdominal segments A (showcasing abdominal prolegs) showcasing a zone lacking Al1. The segments and appendages are labeled for the full view embryos (A, E–F). Full embryo views highlight Antennal (Ant.), eyes, Mandibular (Mn), Maxillary (Mx), and Labial (Lb) head appendage precursors. The 3 pairs of thoracic legs, 4 pairs of abdominal prolegs, and the pair of anal prolegs buds are also marked. Panels show detection of DNA (B,C,D,G), F-actin (B',C',D',G’), Al1 (B'',C'', D’, G’’), and a merge (A, B''',C’’,D'',E–F,G’’’). Yellow dashed lines highlight the contour of appendages showcasing extension defects. White asterisk marked appendages with extension defects within clones lacking Al1. White dashed line highlight -Al1 clones along the embryo body