Fig. 5

Immunodetection of Aristaless1 in al1 CRISPR knockout pupal wings of white Heliconius cydno forewings (3 days APF). A Immunodetection of Al1 in an al1 CRISPR knockout forewing shows Al1 depleted clones. B Closer view of an extensive clone (white dashed line) within the distal edge of the wing. C Side digital reconstruction from a z-stack showing the transition from high Al1 (left, outside the clone) to absent Al1 (right, within the clone). Panels show detection of DNA (C), Al1 (C'), F-actin (C''), and a merge (C''') view. D–E Views of the scale precursors inside and outside of different CRISPR clones. A, B, D, and E show both Al1 and F-actin