Fig. 1

Immunodetection and in situ hybridization for Al1 and Al2 protein and transcript in mid (42–48 h after deposition) wild type Heliconius cydno embryos. A Immunodetection of Al1 and Al2 in a wild-type embryo (lateral-view). B Al1 in situ hybridization. C Al2 in situ hybridization. D, E Details on the immunodetection of Al1 and Al2 in mouthparts (D) and thoracic legs (E) are shown. Panels show detection of DNA (A′, D, E), Al1 (A″, D′, E′), Al2 (A″′, D″, E″), and a merge (A, D″′, E″′). Scale bars are shown on the merged images