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Fig. 3 | BMC Biology

Fig. 3

From: Divergent expression of aristaless1 and aristaless2 during embryonic appendage and pupal wing development in butterflies

Fig. 3

Immunodetection of Al1 and Al2 in early (48–60 h after deposition) wild-type and –Al1 CRISPR Heliconius cydno embryos. A Immunodetection of Al1 and Al2 in a wild-type embryo (lateral-view). B Immunodetection of Al1 and Al2 in a wild-type embryo (dorsal-view). C Al2 in situ hybridization of wild-type embryo (D–G). Immunodetection of Al1 and Al2 in specific sensory appendages (mouthparts [D], abdominal leg tips [E], eye [F], spines [G]). Panels show detection of DNA (D, E, F, G), Al1 (C′, D′, E′, F′, G′), Al2 (C″, D″, E″, F″, G″), and a merge (A, B, C″′, D″′, E″′, F″′, G″′). Scale bars are shown on the merged images

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