Fig. 5

Detailed comparison of the immunodetection of Al1 and Al2 across development in wild-type spines of Heliconius cydno embryos. A Immunodetection of Al1 and Al2 in wild-type embryonic spines 36 (A), 48 (B), 50 (C), and 60 (D) h after deposition. Panels show detection of DNA (A, B, C, D), Al1 (A′, B′, C′, D′), Al2 (A″, B″, C″, D″), and a merge (A″′, B″′, C″′, D″′). Scale bars are shown on the merged images. A schematic representation of the observed subcellular detection for Al1 and Al2 is shown on the right of each time point