Fig. 7

Immunodetection of Al1 and Al2 across pupal development in wild-type Heliconius cydno. Immunodetection of Al1 and Al2 is shown in wild-type pupal wings 1 (A), 3 (B–D), and 4 (E–G) days after pupal formation (APF). For 3 days APF a view of the scale (B) and nuclei (C) z-levels are shown as well as a side reconstruction of the entire scale cell body (D). Similarly, for 4 days APF a view of the scale (E) and nuclei (F) z-levels are shown as well as a side reconstruction of the entire scale cell body (G). Panels show detection of DNA (A, B, C, D′, E, F, G′), Al1 (A′, B′, C′, D″, E′, F′, G″), Al2 (A″, B″, C″, D″′, E″, F″, G″′), and a merge (A″′, B″′, C″′, D, E″′, F″′, G). Scale bars are shown on the merged images