Fig. 3
From: TRPM4 regulates hilar mossy cell loss in temporal lobe epilepsy
Trpm4−/− MCs are more protected after KA injection compared to WT MCs. A Timeline of the experiments. Mice underwent KA or saline (ctrl) injection. Ten days later, behavioral tests were performed to assess spatial and contextual memory (detailed in: Fig. 5). At day 14, mice were sacrificed for histological studies. Representative 10 × confocal images of SATB1immunopositive neurons in the hilus from WT (B) and Trpm4−/− (C) ctrl (left side) and KA (right side) injected mice. Statistics showing the number of MCs in WT and Trpm4−/− mice ipsilateral (D) and contralateral (E) to the site of the injection. n = 3 for WTctrl, 9 for WTKA, 3 for Trpm4−/−ctrl, and 7 for Trpm4−/−KA. Cell numbers are normalized to the saline injected WT group. Scale bar 50 μm. Data are presented as mean ± SD. *p < 0.05, **p < 0.01; ***p < 0.001