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Fig. 1 | BMC Biology

Fig. 1

From: Massive expansion of sex-specific SNPs, transposon-related elements, and neocentromere formation shape the young W-chromosome from the mosquitofish Gambusia affinis

Fig. 1

Comparative genomic hybridization (CGH) using genomic DNA from Gambusia affinis (GAF) and G. holbrooki (GHO) male (m) and female (f) individuals, hybridized to G. affinis metaphases. AD Examples of G. affinis metaphase spreads after CGH using differentially labeled combinations of genomic DNA as indicated. Repetitive elements are enriched in the centromeric regions of all chromosomes. Arrowheads highlight the W-chromosome in female metaphase spreads, with the entire long arm Wq enriched in repeats present in males and females from both species. Asterisks mark a medium sized acrocentric pair in males and females showing a long-arm subtelomeric repeat cluster overrepresented in G. holbrooki. E Summary of genomic imbalances detected by CGH. Genome-wide copy number of repeat sequences is generally higher in G. holbrooki than in G. affinis, but no sex-specific copy number imbalances were observed in the two species (scale bars: 10 µm)

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