Fig. 2

Quantitative chromosome painting in G. affinis using a Z-specific oligopaint probe. A Female (left) and male (right) G. affinis metaphases after co-hybridization of the GAF Z-oligopaint (red) with female genomic DNA (green). Note that the Z-oligopaint hybridizes to the Z- and the W-chromosome, indicating only very limited sequence divergence between Z and W. B enlarged partial metaphase—from left to right depicting the merged image, the Z-oligopaint, and the genomic DNA. C Quantitative fluorescence intensity profiling of the Z-oligopaint (red) and the genomic DNA (green) along the W-chromosome, the Z-chromosome, and an autosome. The top row illustrates the profile of each one individual chromosome from a single representative metaphase, the bottom row shows the mean profiles from ten metaphases. The Z-oligopaint shows higher fluorescence intensity on the Z-chromosome compared to the W. Moreover, oligopainting intensity on the W long arm was dimmed compared with the W short arm. Genomic DNA profiles showed high-intensity values in centromeric regions (CEN) of W, Z, and autosomes, as well as an increased hybridization intensity in euchromatic regions on Wq compared to Wp, Z, and autosomes