Fig. 2
From: MUS81 cleaves TOP1-derived lesions and other DNA–protein cross-links
MUS81-EME1 and TDP1 cannot remove TOP1 in a native state. The substrate bearing native TOP1 covalently bound to the 3′ end of the nick was mixed with the indicated amounts of MUS81-EME1 or TDP1, and the mixture was incubated for 15 min at 37 °C for MUS81-EME1 or at 30 °C for TDP1. Neither MUS81-EME1 (A) nor TDP1 (B) can cleave the native TOP1 substrate. The reaction was stopped with SDS in both cases, and the samples were run on native PAGE