Fig. 5

Depletion of TDP1 sensitises MUS81-KO cells to CPT and affects their proliferation. A The efficiency of TDP1 and TOP1 knockdown in CAL51 WT and MUS81-KO cells was determined by Western blot. Actin was used as a loading reference. B The proliferation of untreated CAL51 WT and MUS81-KO cells with or without TDP1 depletion was measured by WST-1 assay. The means and standard deviations from three independent experiments are shown. The P values were calculated through an unpaired two-tailed t-test (*P < 0.05, ***P < 0.001). Individual data values can be found in Additional file 10. C Cell proliferation of CAL51 WT and MUS81-KO cells simultaneously treated with corresponding siRNA (siCon, siTDP1, or siTOP1) and the presence of increasing CPT concentrations. To maintain the amount of siRNA of the double mutant in all samples, extra siCon was added for single-depleted and control cells. The means and standard deviations from three (WT) and five (KO) independent experiments are shown. The statistical significance was determined by two-way ANOVA test (*P < 0.05, ***P < 0.001). Individual data values can be found in Additional file 10