Skip to main content
Fig. 4 | BMC Biology

Fig. 4

From: Modeling early germline immunization after horizontal transfer of transposable elements reveals internal piRNA cluster heterogeneity

Fig. 4

Conversion of the P(lacW) transgenes by P(lArB) or P(RS3). Diagrams of P(lArB) inserted in cluster 1A (A) and P(RS3) inserted in cluster 100F (B). piRNAs produced by both clusters are represented by small colored lines below the transgenes. Crosses to convert the seven P(lacW) transgenes inserted in tandem by P(lArB) (C) or by P(RS3) (D). Complementary maternal piRNAs produced by either P(lArB) or P(RS3) are indicated above the P(lacW) scheme. Normalized reads of 23–29-nt mapping to lacZ (E, H), white (F, I), and plasmid sequence (G, J). When P(lacW) transgenes are activated by P(lArB), the density of 23–29-nt small RNAs between G1 and G4 is similar for lacZ (E) and the plasmid sequence (G) and 2.25-fold higher for white (red box, F). When P(lacW) transgenes are activated by P(RS3), the density ratio of 23–29-nt small RNAs is close to 1 between G1 and G7 for all the domains of P(lacW) (1.3 for lacZ (H), 0.8 for white (I) and 1.3 for the 1.8-kb plasmid region (red box, J) that is not targeted by maternal piRNAs in G1). The density of normalized 23–29-nt reads per kb (reads/kb) and the fraction of 1U bias at 5′ are indicated in each panel. The P(lArB) (18 kb), P(RS3) (6 kb), and P(lacW) (10.7 kb) transgenes are not drawn to scale

Back to article page