Fig. 5

Chromatin state and steady-state transcription of cluster 1A. A Schematic representation of one of the subtelomeric repeats of cluster 1A of the P-1152 strain, containing the PlArB transgenes (indicated by an asterisk). Yellow arrows indicate the position of qPCR primers. The X subtelomeric repeats (1.8 kb) and the P(lArB) (18 kb) are not drawn to scale. B H3K9me3 ovarian enrichment on three different regions of P(lArB) and of T3 in maternal and paternal inheritance (MI, red; PI, blue) measured by ChIP-qPCR in G1. The signal was normalized to the 42AB region highly enriched in H3K9me3 marks. ChIP experiments were performed on three independent biological samples. P-values were calculated using a bilateral t-test (n = 3). B Ovarian RNA accumulations of G1 P(lArB) and T3 in P-1152 (T3(P-1152)) and T3 in Oregon (T3(Oregon)) were measured by RT-qPCR in control KD (white-KD) and moon-KD and normalized to the expression of RpL32 gene. P-values were calculated using a one-way ANOVA test followed by a Tukey HSD test (n = 3). ns, not significant. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (Additional file 2: Table S8)