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Fig. 1 | BMC Biology

Fig. 1

From: 20-Hydroxyecdysone counteracts insulin to promote programmed cell death by modifying phosphoglycerate kinase 1

Fig. 1

High titers of 20E oppose the insulin effect in regulating glycolysis and repressing PGK1 expression. A Glucose and lactate levels in hemolymph after 20E injection over time and dosage. p < 0.05, n = 3. B Glucose and lactate levels in hemolymph after insulin injection across time and dosages. p < 0.05, n = 3. C qRT-PCR analysis of the downregulation of PGK1 in the larval fat body over time and dosage in response to 20E. p < 0.05, n = 8 or 9. D qRT-PCR detection of PGK1 expression in the larval fat body based on insulin injection over time and dosage. p < 0.05, n = 8 or 9. DMSO or PBS was used as a solvent control for same time. 500 ng/larva of 20E or 5 μg/larva of insulin was injected into sixth-instar larvae at 6 h. Bars indicate means ± SD for more than three independent experiments with five larvae per replicate. Statistically significant differences were calculated using one-way analysis of variance (ANOVA, p < 0.05) tests

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