Skip to main content
Fig. 4 | BMC Biology

Fig. 4

From: 20-Hydroxyecdysone counteracts insulin to promote programmed cell death by modifying phosphoglycerate kinase 1

Fig. 4

PGK1 knockdown in the fifth-instar larvae decreased glycolysis and led to small pupa. A Phenotypes after injection of dsRNA from 5F to 6th-6 h (500 ng/larva administered in the fifth-instar feeding larvae, three times over 24 h intervals). Images were obtained after RNAi exposure for 10 days. dsGFP was used as a negative control. RNAi was performed on three repeats and 30 larvae for each repeat. B Percentages of phenotypes in A. **p < 0.01, n = 3. C The average body weight of pupa after RNAi exposure in A. *p < 0.05, n = 3. D The pupation time from 6th-6 h to pupa 0 d after PGK1 knockdown in A. **p < 0.01, n = 3. E and E’ PGK1 phosphorylation levels in dsGFP and dsPGK1 fat body after the third dsRNA injection. PGK1 proteins from the fat body were purified by CNBr-activated Sepharose 4B with an anti-PGK1 monoclonal antibody. *p < 0.05, n = 3. F Efficiency analysis of PGK1 knockdown at protein levels from the input of E. *p < 0.05, n = 3. G PGK1 glycolytic activity decreased based on the detection of Vmax for 3-PG production in vitro from E. PGK1 proteins from the fat body were purified by CNBr-activated Sepharose 4B with an anti-PGK1 monoclonal antibody, followed by in vitro detection. *p < 0.05, n = 3. H and I The levels of glucose and lactate in H. armigera hemolymph after the third dsRNA injection. *p < 0.05, n = 3. Data represent means ± SD for three independent experiments with five larvae per replicate. Statistically significant differences were calculated using two-tailed Student’s t tests (*p < 0.05 and **p < 0.01). pY, the antibody against phosphorylated tyrosine

Back to article page