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Fig. 2 | BMC Biology

Fig. 2

From: A unique effector secreted by Pseudozyma flocculosa mediates its biocontrol activity

Fig. 2

Knocking out Pf2826 resulted in loss of biocontrol activity. Biocontrol activity of wild type Pseudozyma flocculosa and Cas9 knock-out strains Pf2826AX and Pf282659X were tested against Blumeria graminis f. sp. hordei (Bgh) over time. A Stereomicroscopy and scanning electron microscopy (SEM) observations of the biocontrol activity against Bgh at 36 h post-inoculation (hpi). Arrows show Bgh conidia; arrowheads show P. flocculosa structures. B Biocontrol activity was rated on a scale of 0 to 5 where 0 = no activity and 5 = complete collapse of powdery mildew colonies at 24 and 36 hpi. Each value is the mean of three independent experiments ± standard error (SE). Each experiment comprised five leaves and three different sites per leaf. *p < 0.01; indicates significant difference by Student’s t test (Additional file 6). Black bars = 400 μm; white bars = 25 μm

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