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Fig. 4 | BMC Biology

Fig. 4

From: A unique effector secreted by Pseudozyma flocculosa mediates its biocontrol activity

Fig. 4

Yeast-2-hybrid assays showing the interactions between effector Pf2826 from Pseudozyma flocculosa and candidate proteins from barley and Blumeria graminis f.sp. hordei. Interactions were first named for the bait (BD vector) followed by the prey (AD vector). From top to bottom, candidate interactors are A0A287N0S4 (germin); A0A287RAV7 (HvPR-1a); N1J4Z2 (Sgk2), A0A383V2V0 (Unch-Protein), N1J6Z3 (CSEP0313), A0A383UML4 (Unch-effector), N1J908 (Putati-effector), and F2DJR4 (Chitinase). Log-phase cultures of Y2HGold yeast cells containing plasmids with different combinations were washed and plated at different dilutions on non-selective media (lacking tryptophan and leucine) and selective media (lacking tryptophan, leucine, histidine, and adenine) and incubated at 30 °C for 3 to 5 days. Growth promotion on selective media indicates interaction. Growth in bi-directional cloning indicates strong interaction, while growth in one cloning direction indicates weak interaction. Results shown are representative of those obtained from three independent transformants

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