How does the multifaceted plant hormone salicylic acid combat disease in plants and are similar mechanisms utilized in humans?

Salicylic acid (SA) is an important plant hormone that regulates many aspects of plant growth and development, as well as resistance to (a)biotic stress. Efforts to identify SA effector proteins have revealed that SA binds to and alters the activity of multiple plant proteins—this represents a shift from the paradigm that hormones mediate their functions via one or a few receptors. SA and its derivatives also have multiple targets in animals; some of these proteins, like their plant counterparts, are associated with pathological processes. Together, these findings suggest that SA exerts its defense-associated effects in both kingdoms via a large number of targets.

How was SA's status as a hormone discovered?
The first evidence that SA is a plant hormone came from studies of voodoo lily (Sauromatum guttatum Schott) [6]. During blooming, the voodoo lily inflorescence exhibits two episodes of thermogenesis (heat production). These events increase the surface temperature of the inflorescence by 12 and 10°C and are thought to volatilize compounds that attract/stimulate insect pollinators. Internal SA levels increased~100-fold prior to each episode [7]. Externally supplied SA and two closely related analogs also induced thermogenesis, whereas 31 other SA analogs did not. SA induces thermogenesis primarily by stimulating the mitochondrial alternative respiratory pathway [8]. This pathway, unlike the cytochrome respiratory pathway, generates ATP at just one step and releases the remaining energy from electron flow as heat. Interestingly, SA treatment also induces the expression of alternative oxidase and/or the alternative respiratory pathway in non-thermogenic plant species [9,10].
The year after SA's function in thermogenesis was elucidated, its role as a defense signaling hormone was documented (see below). SA also has long been proposed as a signal for flowering [11]. Consistent with this possibility, the early flowering phenotype observed in several Arabidopsis mutants correlates with, and is dependent on, elevated SA levels [12][13][14][15]. In addition, flowering was delayed in one study of SA-deficient Arabidopsis [16], although others failed to observe this phenomenon [17,18]. Interestingly, several proteins that regulate both flowering and resistance signaling have been identified [12,13,15,[17][18][19][20][21]. While this finding suggests an interconnection between flowering and disease resistance [22], it is clearly complex: some of these proteins regulate both resistance and flowering in an SA-dependent manner, whereas several others regulate resistance via an SA-dependent pathway but positively or negatively regulate flowering via an SA-independent mechanism.

How do plants resist pathogen infection?
Although plants lack the circulating immune cells found in vertebrates, they do possess an innate immune system that detects and limits pathogen colonization [23][24][25]. One branch of this system uses pattern recognition receptors (PRRs) on the plant cell surface to survey for molecules containing characteristic patterns that are unique to, and broadly conserved in, microbes. Detection of these pathogen-/microbe-associated molecular patterns (PAMPs/ MAMPs) leads to activation of pattern-triggered immunity (PTI). In many cases, PTI prevents further pathogen colonization. However, some pathogens have evolved effector proteins that suppress PTI. These pathogens are combatted via effector-triggered immunity (ETI), which comprises the other branch of the innate immune system. ETI is activated when plant-encoded resistance (R) proteins, which are generally located within the plant cell, directly or indirectly recognize their cognate pathogenencoded effectors. Both PTI and ETI are associated with the activation of defenses in the inoculated tissue, including the generation of reactive oxygen species (ROS), increases in intracellular Ca 2+ concentrations, activation of mitogenactivated protein kinases (MAPKs), increased expression of various defense-associated genes, synthesis of antimicrobial compounds and accumulation of SA [26,27]. Generally, ETI induces these defenses more rapidly and intensely than PTI. ETI also is usually associated with necrotic lesion formation, which may help restrict pathogen movement from the infection site. Subsequent to these events, ETI and PTI can induce immune responses in the uninoculated (systemic) portions of the plant, including a long-lasting, broad-spectrum resistance called systemic acquired resistance (SAR) [2,23,28].
How was SA's role as a hormone signaling disease resistance discovered?
Since the late 1970s, it was known that applying SA to tobacco plants induces defense gene expression and enhances resistance to viral infection [29]. However, SA's role as an internal signal for disease resistance was not demonstrated until 1990, when rises in SA levels were detected prior to the development of local and/or systemic disease resistance in tobacco and cucumber [30,31]. Analyses of tobacco and Arabidopsis unable to accumulate SA (due to various mutations or expression of SA-degrading enzymes) confirmed that SA is required for PTI, ETI, and SAR [2]. Grafting studies using SA-deficient or wild-type (wt) tobacco further indicated that while SA accumulation is required in the uninfected leaves for SAR development, SA is not the mobile SAR-inducing signal that travels from the inoculated to systemic leaves [32,33]. SA's role as a defense signal has been extended to many plant species. However, there are conflicting reports regarding its role in some monocots [2], as well as in plants that constitutively accumulate high levels of SA (such as potato and rice); its role in some plant species also appears to vary depending on the pathogen [34][35][36][37].
It should be noted that SA is one of several plant hormones involved in signaling defenses against microbial pathogens [38,39]. The SA-mediated defense signaling pathway is activated following infection by biotrophic pathogens, which require living host tissue. By contrast, attack by necrotrophic pathogens, which feed on dead tissue, induces a distinct defense pathway that is regulated by the plant hormones jasmonic acid (JA) and ethylene. The SA-and JA/ethylene-mediated defense pathways undergo extensive cross-talk; their interactions are generally antagonistic.

How do plants synthesize SA?
Plants utilize the isochorismate (IC) and the phenylalanine ammonia-lyase (PAL) pathways to synthesize SA, as well as many other important compounds ( Fig. 1; for more information, see [40]). Although neither route for SA biosynthesis is completely understood, both are known to require the primary metabolite chorismate [40][41][42]. In the PAL pathway, PAL converts phenylalanine (Phe) to trans-cinnamic acid (t-CA). Depending on the plant species, t-CA is converted to SA via the intermediates ortho-coumaric acid or benzoic acid (BA). The conversion of BA to SA presumably occurs via BA 2hydroxylase. The IC pathway was identified based on the hypothesis that plants synthesize SA via a pathway analogous to that of some bacteria [43]. Indeed, genes encoding isochorismate synthase (ICS), which converts chorismate to isochorismate, have been identified in many plant species. However, no plant gene corresponding to bacterial isochorismate pyruvate lyase, which converts isochorismate to SA and pyruvate, has been identified. Following its synthesis, Arabidopsis ICS1 is imported to the chloroplast stroma, where SA synthesis occurs [44].
Demonstrating the IC pathway's importance, Arabidopsis ics1 mutants (also designated SID2 or EDS16) accumulate 90-95% less pathogen-induced SA and display decreased disease resistance [43,45,46]. Similarly, pathogen-induced SA was suppressed in ICS-silenced tomato and Nicotiana benthamiana [47,48]. Furthermore, diverse pathogens secrete chorismate-or isochorismate-metabolizing enzymes that promote virulence by suppressing plant SA accumulation [49,50]. Since Arabidopsis mutants defective for both ICS genes lack ICS activity but accumulate low SA levels [51], an alternative, presumably PAL-dependent, pathway must also be functional. Indeed, PAL-deficient Arabidopsis, tobacco, and pepper exhibited reduced pathogeninduced SA accumulation and disease resistance, whereas Arabidopsis overexpressing CaPAL1 from pepper displayed increased pathogen-induced SA accumulation and resistance [33,52,53]. It should be noted, however, that pathogen-induced SA accumulation still occurs in PALsuppressed plants, while it is largely abrogated in ICSdeficient plants. Whether PAL suppression affects SA accumulation by impacting the phenylpropanoid pathway or via an indirect effect on SA precursors, such as chorismate, is unclear [52].
Interestingly, a chloroplast-localized, calcium-sensing receptor (CAS) is required for full activation of various ETI-/PTI-induced defenses, including SA accumulation, stromal Ca 2+ fluxes, stomatal closure, nuclear-encoded defense gene expression, and pathogen resistance [67]. Although the mechanism(s) through which CAS exerts its effects is unclear, these findings suggest that retrograde signals from chloroplasts also regulate SA biosynthesis and immunity [27].
How are cytosolic SA levels regulated?
After synthesis in the chloroplast, SA is transported to the cytosol where it signals immune responses. In Arabidopsis, SA export is likely mediated by EDS5 (also designated SID1), a chloroplast envelope-localized member of the multidrug and toxin (MATE) transporter family [68,69]. Once in the cytoplasm, SA can undergo various modifications that generally render it inactive (Fig. 2) [40,41,58]. These modifications are thought to help regulate the level of biologically active SA in the cytoplasm, provide a rapidly accessible source of SA and/or facilitate SA transport throughout the plant. Maintaining low cytoplasmic SA levels except during immune signaling is critical, as The level of biologically active SA in the cytoplasm is regulated by an array of modifying enzymes that convert it to biologically inactive derivatives (reviewed in [40]). Shown here are the structures of SA and its derivatives; their interconversions are indicated by solid arrows. The prodrugs salicin, which is a natural salicylate, and aspirin, a synthetic SA derivative, are also depicted; their conversion to SA in animals is indicated by broken arrows. Methyl salicylate (MeSA) is both a transported form of SA in planta and a prodrug in humans; thus, the in planta conversions are depicted by solid arrows, while the human conversion of the prodrug is represented as a dashed line. Question marks indicate either that the reaction steps have not yet been identified or that the enzyme responsible for the indicated conversion has not yet been definitively identified. In animals, aspirin is rapidly converted to SA with a half-life (t1/2) of approximately 20 min constitutive activation of immunity reduces plant fitness by shunting both energy and resources away from growth and reproductive processes [70]. Glucosylation of SA at its hydroxyl group generates SA 2-O-β-D-glucoside (SAG), while glucosylation at its carboxyl group produces salicylate glucose ester (SGE). SAG is transported to the vacuole, where it serves as a non-toxic storage form that can be hydrolyzed to release free SA following pathogen attack. Methylation of SA generates methyl SA (MeSA). MeSA is a phloem-mobile SAR signal that travels from the infected leaf to the systemic tissues, where it activates resistance following its conversion back to SA [71]. Pathogeninfected Arabidopsis also accumulate 2,3-dihydroxybenzoic acid (2,3-DHBA) and, to a lesser extent, 2,5-DHBA [72]. Conversion of SA to 2,3-DHBA is mediated by SA 3hydroxylase (S3H; also termed DLOL1) [73,74]. Since s3h mutants accumulate high levels of SA, S3H-mediated hydroxylation may be a critical mechanism for preventing SA over-accumulation [73]. Formation of SA-amino acid conjugates is yet another modification strategy [40]. Salicyloyl-aspartate (SA-Asp) has been identified in plants; it is presumably synthesized by GH3.5 (also termed WES1), a member of the GH3 acyl adenylase family [75]. Another GH3 family member, GH3.12 (also termed PBS3, GDG1, and WIN3), conjugates 4-substituted benzoates to Glu [76]. Since gh3.12 mutants display reduced pathogeninduced SA/SAG accumulation and disease resistance, and these phenotypes were rescued by SA, GH3.12 was predicted to function upstream of SA synthesis/accumulation [40]. Given that stress-treated gh3.12 mutants accumulate elevated levels of SA-Asp, GH3.12 may increase cytosolic SA levels by repressing SA-Asp synthesis.

What plant processes does SA affect?
In Arabidopsis, the basal level of total SA (consisting of SA and SAG/SGE) in leaves ranges from 0.22-5 μg/g fresh weight; in the Solanaceous species tobacco and potato it is <0.4 μg/g fresh weight and <17 μg/g fresh weight, respectively [77]. Given the wide range in basal SA levels between (and even within) plant species, it is perhaps not surprising that conflicting reports have been published concerning the effect of exogenously supplied SA on various plant processes. Despite this caveat, exogenous SA has been shown to affect resistance to biotic (pathogen-associated) stress and tolerance to many abiotic stresses (drought, chilling, heat, heavy metal, UV radiation, and salinity/osmotic stress), as well as multiple aspects of plant growth and development, including seed germination, vegetative growth, flowering, fruit yield, senescence, thermogenesis, stomatal closure, root initiation/growth, photosynthesis, respiration, glycolysis, Krebs cycle, and the alternative respiratory pathway [42,[78][79][80][81]. Some of these processes are induced by SA in a concentration-dependent manner, as they are activated by treatment with a low dose of SA and inhibited by a high dose. This phenomenon is likely linked to SA's role in regulating cellular redox status, as low concentrations of SA induce low-level accumulation of ROS, which serve as secondary signals to activate biological processes. By contrast, high concentrations of exogenous SA stimulate the accumulation of high levels of ROS, which cause oxidative stress and cell death [2]. Currently, the mechanisms through which SA regulates these non-immune plant processes are not well understood, but they do appear to involve the coordinated effect of SA and other plant hormones.

How does SA work-a few receptors or multiple targets?
It is generally assumed that plant and animal hormones signal downstream responses by binding to one or a small number of receptors. Whether this paradigm can be extended to SA is currently unclear. In Arabidopsis, members of the non-expressor of pathogenesis-related genes (NPR) protein family were proposed to be SA receptors [82][83][84]. SA binding to NPR1 (also designated NIM1 and SAI1) may promote immune signaling by relieving sequestration of NPR1's trans-activating domain (see below) [83]. By contrast, NPR3 and NPR4 appear to be negative regulators of immunity [82]. Identification of these putative SA receptors represents progress. However, several lines of evidence argue that additional SA-binding proteins (SABPs) mediate SA's functions. First, some immune responses are activated via an SA-dependent but NPR1independent pathway(s). Second, there is little evidence that non-immune processes affected by SA are NPR1dependent. Indeed, nearly 30 SABPs have been identified; these proteins exhibit a range of affinities for SA and their activities are altered by SA binding [77,85]. Given that SA levels can vary dramatically between plant species and also within a plant, depending on its developmental stage, the tissue type, subcellular compartment, and exposure to (a)biotic stress, it is possible that SA signals its many effects by interacting with different SABPs, depending on their location and SA affinity [77]. This hypothesis represents a paradigm shift for how at least some hormones function. We recently proposed that proteins whose activity is altered following hormone/ligand binding be termed "targets", and only a subset of targets that meet additional criteria be designated "receptors". Determining which criteria must be met to qualify an SABP as an SA receptor will be complicated since i) SA binding alters the activity of many SABPs and ii) some SABPs exhibit comparably high affinity as the proposed receptors NPR1 and NPR4, while others display a low affinity similar to NPR3.
What is the best-defined SA signaling pathway? SA signals defense responses via both NPR1-dependent and NPR1-independent pathways; of these, the NPR1-dependent pathway is the best understood. Arabidopsis npr1 mutants fail to activate immunity following SA treatment or pathogen infection, indicating NPR1's critical importance for SA signaling [41,58]. NPR1 contains two domains mediating protein-protein interactions: a BTB/ POZ and an ankyrin repeat domain, as well as a Cterminal trans-activating domain and nuclear localization sequence [86]. In uninfected plants, NPR1 is predominantly sequestered in the cytosol as oligomers. After infection, SA-induced changes in the cellular redox state reduce NPR1 to monomers that are transported to the nucleus. There, NPR1 co-activates transcription of immuneassociated genes [87]. For the defense marker gene pathogenesis-related-1 (PR-1), NPR1 directly interacts with members of the TGA transcription factor family [2,26,88]. PR-1 expression may be further regulated by interactions between NPR1 and members of the non-inducible immunity 1 (NIM)-interacting (NIMIN) family, and by proteins proposed to function downstream of NPR1 [26,88]. NPR1 also upregulates the expression of WRKY TFs, which regulate many immunity-associated genes [89]. WRKY binding sites are located in the NPR1 and ICS1 promoters, suggesting the presence of a feedback loop that fine-tunes SA signaling [26].
To prevent spurious/inappropriate induction of immune responses, NPR1 activity is tightly regulated. Besides nuclear translocation, SA binding at two cysteine residues (Cys521 and Cys529) may regulate NPR1 activity by inducing a conformational change that releases the transactivating domain from the inhibitory BTB/POZ domain [83]. How this mechanism applies to plant species whose NPR1 homologs lack Cys521 and Cys529 is unclear [86,90]. NPR1 activity also is regulated via proteasome-mediated degradation [91]. This process may be mediated by NPR3 and NPR4, which are adaptors for Cullin 3 ubiquitin E3 ligase [82]. NPR4 is proposed to maintain low NPR1 levels in uninfected cells. Following infection, SA disrupts the NPR1-NPR4 interaction, allowing NPR1 to accumulate and defense signaling to occur. In cells containing sufficiently high SA levels, NPR3 binds NPR1; this promotes NPR1 turnover, which optimizes defense activation and resets NPR1 levels [82,91].

How is SA research benefitting agriculture?
Synthetic pesticides have allowed growers to dramatically increase crop yield and quality. However, these compounds are often toxic and their overuse has led to pathogen resistance [92,93]. An environmentally friendlier strategy for reducing crop loss involves regulating SAR [94]. SA is an effective SAR inducer, but its phytotoxicity precludes widespread use [95]. Several synthetic compounds (Fig. 3), including 2,6-dichloroisonicotinic acid (INA), benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (also termed benzothiadiazole; BTH or acibenzolar-S-methyl), and probenazole (PBZ) and its active metabolite 1,2-benzisothiazol-3(2H)-one 1,1-dioxide (BIT), induce defense gene expression and SAR to a similar range of pathogens as SA [96,97]. PBZ and BIT activate SAR by triggering the SA signaling pathway upstream of SA [98], whereas INA and BTH are SA functional analogs [96,99]. While treating plants or suspension cells with high concentrations of SA or its functional analogs directly induces defenses, low concentrations elicit little to no response. Following subsequent infection, however, defenses are activated more rapidly and/or strongly [100]. This phenomenon, termed priming, also occurs in systemic leaves of plants exhibiting SAR. Although not fully elucidated, the molecular mechanisms of priming likely involve the accumulation of transcripts and/or inactive forms of MAPKs, elevated levels of PRRs, and chromatin remodeling [95]. This latter mechanism may also promote the inheritance of defense priming.
In addition to SA, its functional analogs and PBZ/BIT, other resistance-activating compounds that work at least in part by inducing/priming SAR, have been identified [96,97,101]. In the field, resistance triggered by SAR inducers/primers can be broad-spectrum and long-lasting, but it is rarely complete; disease reduction ranges from 20-85% [97]. Other drawbacks growers must currently consider include i) the potential for reduced plant fitness, which may be minimized by stimulating priming versus direct induction [102], ii) variable efficacy, depending on plant cultivar and dosage, and iii) the possibility that SAR inducers will increase susceptibility to necrotrophic pathogens, due to suppression of the JA signaling pathway [96,97]. Nevertheless, future efforts to clarify the SA signaling pathway should provide insights into how current SAR-inducing compounds can be improved, as well as identify novel pathway components that could be targeted by the next generation of agrochemicals.
How did aspirin become a wonder drug?
Plants rich in SA and its derivatives, collectively termed salicylates, have been used for medicinal purposes for millennia. In the fourth century BC, willow leaves/bark extracts were prescribed by Hippocrates to relieve fever and the pain of childbirth [2]. Salicylate-rich plants were also used by other ancient cultures, including the Babylonians, Assyrians, and Chinese, as well as the indigenous inhabitants of the New World [42]. Although willow bark was a well-known folk remedy, its medicinal effects were first studied clinically in the mid-1700s by the Reverend Edward Stone [3]. In 1828, Johann Buchner purified the active ingredient in willow bark and named it salicin (Fig. 2). Raffaele Piria subsequently demonstrated that salicin could be split into a sugar and an aromatic compound he named SA, in reference to Salix alba, the Latin name for white willow [77]. Around this time, high levels of salicylates were detected in other medicinal plants, such as meadowsweet, which contains both salicin and MeSA-then called oil of wintergreen [3,77]. These "prodrugs" are converted to SA after digestion in humans/ animals [77]. Increased demand for SA in the mid-1800s led to the commercial production of synthetic SA in 1874. As SA's price fell and its availability increased, its clinical use expanded. However, SA's negative side effects, particularly stomach irritation, precluded long-term, high-dosage use. Research by Felix Hoffmann revealed that acetylation improved SA's tolerability without affecting its medicinal properties [3]. Bayer and Company began synthesizing acetyl SA (ASA) in 1897 under the trade name aspirin, which was generated by combining the "a" from acetyl and "spirin" from the Latin name for meadowsweet (Spiraea ulmaria). Today, aspirin is one of the most widely used drugs in the world. In addition to treating fever, swelling, pain, and inflammation, aspirin is used prophylactically to reduce the risk of stroke, heart attack, and certain cancers [3][4][5]. What are SA's targets in humans?
The combined observations that i) aspirin irreversibly inhibits the cyclooxygenases COX1 and COX2, ii) these enzymes convert arachidonic acid into prostaglandins, and iii) prostaglandins induce fever, pain, inflammation, and swelling underpin the prevailing assumption that aspirin works via COX1 and COX2 inhibition [103]. However, aspirin is rapidly metabolized to SA in the human body, and SA is a weak COX1/COX2 inhibitor. Given the similarities between SA's and aspirin's pharmacological effects, other SA targets likely exist [77]. Indeed, 13 additional potential targets of salicylates have been identified. The SA/aspirin levels required to alter most of these putative targets' activities are near or above toxic levels. In contrast, low micromolar SA levels in vivo alter the disease-associated activities of high mobility group box 1 (HMGB1) [104] and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) [105]. HMGB1 is an abundant chromatin-associated, non-histone protein that regulates nucleoprotein complex assembly and chromatin condensation [106]. It also is a damage-associated molecular pattern (DAMP) that triggers inflammation and induces cytokine expression when released extracellularly after tissue damage or necrosis. SA binding blocks HMGB1's pro-inflammatory activities [104]. Notably, low SA levels, while unable to inhibit COX1/COX2 enzymatic activity, suppress HMGB1-mediated induction of Cox2 expression.
The glycolytic enzyme GAPDH is another protein whose secondary functions are affected at low SA concentrations. GAPDH likely plays a role in neurodegenerative diseases via a cell death cascade that involves GAPDH, nitric oxide, and the E3 ubiquitin ligase Siah [107]. SA binding suppresses GAPDH's ability to translocate to the nucleus and induce cell death [105]. Additionally, GAPDH enhances hepatitis C virus replication and/or translation by binding the viral genome; SA binding to GAPDH suppresses this activity [77]. Given that SA binding also suppresses the ability of cytosolic Arabidopsis GAPDH to promote plant virus replication [108] and the DAMP activity of HMGB3, an Arabidopsis counterpart to HMGB1 [109], plants and animals share at least several diseaseassociated SA targets.

Why might humans have so many SA targets?
SA is present in the urine and/or blood of humans who have not recently consumed salicylate drugs and also in a wide range of animals [110]. Since humans on a vegetarian diet contain greater levels of SA and its metabolite salicyluric acid than non-vegetarians, consuming large quantities of plants-and their associated salicylatesappears to influence SA levels to some extent [111]. However, no correlation between blood SA levels and diet was observed in herbivorous versus carnivorous animals [110]. The presence or absence of gastrointestinal bacteria also did not affect serum SA levels. Instead, radiotracer studies suggested that SA is synthesized endogenously in humans using BA as a precursor [110]. BA, in turn, may be synthesized endogenously from phenylalanine or provided by the diet, since some fruits and vegetables contain high concentrations of BA and its salts. We previously hypothesized that the presence of low SA levels in animals, whether endogenously synthesized or provided by the diet, led to the evolution of multiple SA targets [77]. Given the myriad effects of SA's prodrug aspirin, others have speculated that SA is a critical animal bio-regulator that plays a key role in orchestrating defense responses, analogous to its role in plants [110]. If these possibilities are confirmed, future studies will likely identify additional SA targets that are shared in plants and animals.
What are the future directions of SA research? Despite significant progress in elucidating the SA signaling pathway for plant disease resistance, significant knowledge gaps remain. Our understanding of the mechanism(s) through which pathogen perception is transduced by PRRs and R proteins into activation of early cellular responses and SA synthesis is fragmentary. Likewise, the enzymes involved in SA biosynthesis are not fully identified and the role of the ICS versus PAL pathways in different plant species remains unclear. The mechanisms through which ICS1 expression is regulated, both in the nucleus and via retrograde signals from the chloroplast, also need to be determined. As for the signaling pathway downstream of SA, a crucial line of study will involve identifying SA targets/receptors and assessing their function. These analyses, combined with the development of in vivo SA detection techniques, should provide tremendous insight into how SA exerts its myriad effects. Elucidation of the SA signaling pathway should benefit agriculture by suggesting strategies for improving current SAR-inducing compounds, as well as facilitating development of novel compounds that target currently unidentified pathway components. Finally, the discovery that animals contain multiple SA targets (which may have emerged in response to low-level exposure to endogenously synthesized and/or dietary SA) opens new avenues for treating human diseases. Indeed, SA-mediated inhibition of HMGB1's pro-inflammatory activities might explain the protective effects of low-dose aspirin use. The identification of natural and synthetic SA derivatives that suppress HMGB1's and GAPDH's disease-associated activities more effectively than SA suggests there is great potential for developing SA-based drugs that are even more efficacious and/or have fewer negative side effects [77]. In summary, it is highly likely that SA's targets in animals and plants will significantly overlap. Characterizing these proteins should not only clarify how SA induces its effects in both kingdoms, but also suggest novel strategies for controlling pathological processes.