Figure 4From: Detailed interrogation of trypanosome cell biology via differential organelle staining and automated image analysisColor deconvolution can separate kinetoplast and nuclear DNA signal in Trypanosoma brucei even when the organelles are closely apposed or abnormal in structure. 4', 6-Diamidino-2-phenylindole (DAPI) and propidium iodide (PI) fluorescence and color-deconvolved images of procyclic T. brucei 48 h after induction of expression of sister chromatid cohesion protein 1 (SCC1)-mutAB. (A) 1K1N cell. (B) 2K2N cell. Signal from the closely apposed anterior kinetoplast and posterior nucleus (arrowhead) can be separated. (C) Zoid (1K0N) cell illustrating a lack of nuclear DNA. (D) Cell with zoid-like DAPI staining which possess a fragment of nucleus next to the kinetoplast (arrowhead). (E,F) Cells undergoing aberrant cytokinesis, having failed to undergo mitosis, with a severely distorted nuclear structure. Color deconvolution can unambiguously identify kinetoplasts (arrowheads) among nuclear DNA fragments. (G) An out of focus 2K1N cell; kinetoplast and nucleus signal can still be accurately separated by color deconvolution despite being out of focus. Scale bar represents 5 μm.Back to article page