Figure 7From: Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23Endocytic defects in awd mutant cells. Notch trafficking assay was performed on wing discs from (A, C) yw; en2.4-Gal4e22c, UAS-flp/+; Ubi-GFP, FRT82B/FRT82B, awdj2A4 and (B) yw third-instar larvae. (A) In a disc containing awd mutant clone (GFP-negative cells) without synchronized induction of endocytosis (steady state time 0), Notch is seen over-accumulating on or near the cell surface. (B) In wild-type (yw) discs, at 20 minutes after initiation of endocytosis, Notch is expressed at a low level in punctates that are mostly localized in Avl-positive vesicles with some localization in Rab7-positive vesicles (arrows). At one hour after initiation of endocytosis, Notch is barely detectable. (C) In awd mutant clones, at 20 minutes after initiation of endocytosis, Notch over-accumulates exclusively in Avl-positive vesicles but not in Rab7-positive vesicles. This pattern persists at one hour after initiation of endocytosis. The accumulated Notch at this time does not overlap with Rab7-positive vesicles (insets). Bars are 5 μm.Back to article page