Figure 8

GFP-positive central nervous system cells of an olfactory marker protein-τGFP reporter mouse line . Double immunostaining for GFP (left panels; green) and Gαo (right panels; red) in brain slices of reporter mouse line OMPCre-eRosa26τGFP. In these mice, olfactory marker protein (OMP) expression enables Cre-mediated recombination of a stop codon preceding the τGFP locus and therefore driving continuous expression of GFP in all OMP-positive cells. GFP staining can be found in the paraventricular nucleus (A), medial preoptic area (B), and medial amygdala (C) of adult female mice. Double staining showed no overlap between GFP and Gαo (arrowheads). Scale bar, 100 μm. GFP, green fluorescent protein.