|  |  | [3H]DA uptake | DAT density |  |
---|
Group | N | Vmax | SD | Km | SD | DAT | SD |
---|
WT w Veh | 4 | 516.1 | ± 56.5 | 56.7 | ± 18.6 | 10.6 | ± 2.5 |
D3 KO w Veh | 4 | 212.4 | ± 18.2 | 10.8 | ± 5.7 | 10.8 | ± 1.5 |
WT w PPX | 6 | 183.4 | ± 34.3 | 28.4 | ± 18.5 | 9.9 | ± 3.0 |
D3 KO w PPX | 6 | 223.6 | ± 36.4 | 10.4 | ± 10.9 | 9.4 | ± 2.6 |
- Wild-type and D3 KO mice were administered vehicle (0.9% saline) or pramipexole (PPX, 0.1 mg/kg I.P.) once a day for 5 days. Twenty-four hours after the last injection the brains were removed, the CPu dissected and fresh frozen ([3H]DA uptake) or brains frozen on dry-ice ([125I] RTI-55 autoradiography).
- [3H]DA uptake Vmax reported as pmol/mg P/5 min and Km reported as nM. [125I] RTI-55 autoradiography for DAT density reported as fmol/μg P.