Figure 8 From: Acetylation of insulin receptor substrate-1 is permissive for tyrosine phosphorylation IRS-1 ubiquitination is not altered by acetylation. MCF-7 cells were treated with PMA, TSA and insulin as described for Figure 4 and immunoprecipitated IRS-1 was western blotted for the presence of ubiquitin (upper panel) and IRS-1 (lower panel). The molecular mass of the immunoprecipitated ubiquitinated protein and IRS-1 protein is indicated.Back to article page