Figure 4

Failure of SPCC-cortical association and pseudocleavage furrow formation in gna-2(qa705) , chs-1(RNAi) and cej-1+B0280.5(coRNAi). Columns of images are a developmental series of individual wild-type, gna-2(qa705), chs-1(RNAi) or cej-1+B0280.5(coRNAi) embryos, from meiosis II metaphase to pronuclear meeting. In wild type, the sperm pronucleus (sp) touches the cortex (indicated by a red arrow) and a pseudocleavage furrow can be seen (indicated by white arrows) before and during pronuclear migration. In gna-2(qa705), chs-1(RNAi) or cej-1+B0280.5(coRNAi) a sperm pronucleus (sp) decondenses but fails to associate closely with the cortex (indicated by a red arrow), and a pseudocleavage furrow was not seen. Maternal (ma) DNA (11–12 sister chromatid pairs can be seen at meiosis II metaphase in gna-2(qa705) and chs-1(RNAi)). Images are of live embryos expressing histone H2B::GFP, recorded in utero.