Figure 3

The acetylcholinesterase inhibitor, aldicarb, does not induce significant tail curling in hermaphrodites. (a) Ventral and (b) dorsal curling in hermaphrodites (25 mM, N = 20 for time points 0 to 15; N = 19 for time points 20 to 30; 10 mM, N = 19 for time points 0 to 15; N = 18 for time points 20 to 30; 5 mM, N = 21; 1 mM, N = 25 for time points 0 to 20; N = 23 for time points 25 to 30; 0.5 mM aldicarb, N = 19; ddH20, N = 40). (c) Ventral and (d) dorsal tail curling in males assayed on the same day as hermaphrodites (25 mM, N = 20; 10 mM, N = 20; 5 mM, N = 20; 1 mM, N = 21; 0.5 mM, N = 20; ddH20, N = 38). Error bars indicate +/- standard error of the mean. Males show a significantly higher amount of ventral tail curling at several time points. Males show a significantly higher amount of dorsal tail curling versus hermaphrodites in aldicarb at early time points: 25 mM/0 min, 1 mM/5 min, P < 0.05; 10 mM/0 min, P < 0.005; 5 mM/0 min,2.5 min, 1 mM/2.5 min. P < 0.0005).